| Abstract |
Background: Arthritis is chronic disabling joint disease. Drugs available produce serious adverse effects on chronic use. Active Ingredient of pineapple fruit (Bromelain) appears promising adjuvant to anti-arthritic drugs. Objective: Evaluation of efficacy of pineapple juice in animal models of rheumatoid arthritis and osteoarthritis. Materials and Methods: First total 48 rats of either sex weighing 250-300 g were divided into 2 batches, (each containing 24 rats), batch I for osteoarthritis and batch II for rheumatoid arthritis . Each batch was subdivided in to 4 groups (n=6). Rheumatoid Arthritis induced by injecting 0.1ml Complete Freund\'s Adjuvant in sub plantar region of left hind paw. 24 Rats divided into 4 groups (n=6). Group-I: Control, Group-II: Pineapple juice, Group-III: Diclofenac, Group-IV: Pineapple juice + Diclofenac. Oral drug treatment was given from day0 to day12. Paw volume was measured by plethysmometer on day 0, 1, 5, 12, 21. Osteoarthritis induced by intra-articular injection of 50 μl Monosodium-Iodo-Acetate solution. 24 rats divided into 4 groups (n=6) as above and drug treatments given for 14 days. Animals were observed for joint inflammation and gait. On day 15 they were sacrificed, knee joints observed for gross changes and sent for histopathology. Results: Localized inflammatory reaction developed in all rats in 24 hours. In control group, no resolution of swelling, while in others, significant anti-inflammatory activity was seen which was maximum in combination Group-IV, followed by Diclofenac and least with Pineapple juice. Similarly significant anti-inflammatory activity was observed in terms of low histopathology scores in all 3 treatment groups of Osteoarthritis model. Conclusion: Results suggest that Pineapple juice has weak anti- inflammatory activity and can be added as a dietary adjuvant to standard anti-inflammatory drugs.
KEYWORDS: Pineapple, Rheumatoid arthritis, Osteoarthritis
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